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Tutorials

This tutorial shows how to run a image-based profiling pipeline using pycytominer. Using IPython notebooks, it walks through the following steps:

  • Downloading a dataset of single cell CellProfiler profiles.
  • Processing the profiles using PyCytominer. This includes the following steps:
  • Data initialization
  • Single cell aggregation to create well-level profiles
  • Addition of experiment metadata to the well-level profiles
  • Profile normalization
  • Feature selection
  • Forming consensus signatures
  • Evaluating the profile quality using cytominer-eval.